Optogenetic stimulation with no strings attached
The Teleopto wireless optical stimulation system is the ideal solution for activating opsins in freely behaving mouse or rat. It provides the user with a combination of the typical colours for opsin activation. An Infrared enabled remote controller sends stimulation pulses to a light-weight (1-4g) head-mounted receiver.
The system comprises a selection of fiber optic cannulas. Each cannula connects to a rechargeable battery-containing receiver – removable when not needed. The fiber optic cannula has one or two built-in miniature LEDs, coupled into one or two optical fibers on the other end. These can be inserted into the tissue.
The receiver, which powers up the miniature LED/s in the fiber optic cannula during stimulation, interprets the IR signal from the remote controller. The remote controller should be positioned near the receiver (within 1m, or within 3m if used with an IR emitter – bidirectional). It accepts trigger inputs from an external stimulator source. The frequency of the TTL inputs synchronizes the frequency of the light pulses.
The Teleopto wireless optical stimulation is the perfect equipment for optogenetics manipulation in freely moving animals. If you are also interested in imaging the brain tissue, please have a look at our other solutions, our Pryer Endoscopic Objective and the OASIS Implant by Mightex Systems.
If you are looking into using custom stimulations for your research protocol, the TeleOpto Stimulator would be of great benefit to you. Please see the section Stimulator for more details or give us a call at +44 1825 890 858.
The Teleopto wireless optical stimulation standard set consists of:
- 3 x LED fiber optic cannulas
- 1 x Teleopto receiver
- 1 x remote controller
- 1 x infrared emitter (for increasing the range of the remote controller)
- 1 x Teleopto charger (for charging the battery on the receiver)
- 1 Cannula insertion tool
- 1 dummy receiver (for animal habituation)
- 1 trigger cable (for connection between the remote controller and an external trigger source)
Now also available: The TeleHub (6 channel infrared emitter) and a Stimulator (pulse generator)
Meet the NEW STO mk-II pulse generator developed for optogenetics.
By connecting the STO mk-II to the TRG port on the Teleopto Remote Controller via a trigger cable, you can control the timing of light stimulation by TTL pulses.
Pulses are defined by the parameters as shown in the image below:
If you would like to learn more about this fantastic new product, please get in touch now.
NEW: The 6 channel TeleHub forms part of the TeleOpto range.
This product can be connected to the EXT Port on the TeleOpto Remote Controller to provide you with 6 infrared emitters simultaneously.
Some of the exciting features of this new hub is, that:
- You will have an increase in throughput of your experimental protocol
- With several infrared emitters in different positions, it ensures stable light stimulation
- The TeleHub is also ideal for use with mazes with many branches or high walls, that effectively block infrared signals and prevent signal transmission
*Please note that all infrared emitters will send a signal at the same time.
We would love to discuss this NEW product with you, so please fill out our contact form and we promise to contact you shortly.
Available cannula options
The default option will be blue/10mm/250um
A thicker fiber means more light will reach the target area if all other variables remain the same, but it is more invasive. As a guideline, the following intensities can be achieved:
The various Teleopto wireless optical stimulation receivers allow the user to select between options of:
- Weight/size/battery life
- Single or two independent channels
- Pulse mode or continuous mode. This affects how the receiver interprets the signal from the remote controller:
The default option for the receiver is 2g and releases a pulsing signal rather than a continuous one.
Please find below a selected list of publications involving the Teleopto wireless optical stimulation system:
Lucy K. Bicks, Kazuhiko Yamamuro, Meghan E. Flanigan, Julia Minjung Kim, Daisuke Kato, Elizabeth K. Lucas, Hiroyuki Koike, Michelle S. Peng, Daniel M. Brady, Sandhya Chandrasekaran, Kevin J. Norman, Milo R. Smith, Roger L. Clem, Scott J. Russo, Schahram Akbarian & Hirofumi Morishita
Nat Commun 11, 1003 (2020)
Izawa, S., Chowdhury, S., Miyazaki, T., Mukai, Y., Ono, D., Inoue, R., … & Terao, A.
(2019) Science, 365 (6459), 1308-1313.
(2019). Doctoral dissertation.
Fu, O., Iwai, Y., Kondoh, K., Misaka, T., Minokoshi, Y., & Nakajima, K. I.
(2019). Cell reports, 27(6), 1650-1656.
Yoshifumi Abe, Yuji Komaki, Fumiko Seki, Shinsuke Shibata, Hideyuki Okano, Kenji F. Tanaka
Neurochem Int. 2019 May; 125:163-174. DOI: 10.1016/j.neuint.2019.02.017.
Risa Sueda, Itaru Imayoshi, Yukiko Harima, Ryoichiro Kageyama
Genes Dev. 2019 Mar 12 DOI: 10.1101/gad.323196.118.
Jiaojiao Song, Da Shao, Xinli Guo, Yanfang Zhao, Dongyang Cui, Qianqian Ma, Huan Sheng, Lan Ma, Bin Lai, Ming Chen, Ping Zheng
Science Advances 2019 Feb 20;5(2):eaat3210. DOI: 10.1126/sciadv.aat3210.
Synchronized activation of striatal direct and indirect pathways underlies the behavior in unilateral dopamine-depleted mice.Synchronized activation of striatal direct and indirect pathways underlies the behavior in unilateral dopamine-depleted mice.
Omar Jáidar, Luis Carrillo-Reid, Yuko Nakano, Violeta Gisselle Lopez-Huerta, Arturo Hernandez-Cruz, José Bargas, Marianela Garcia-Munoz, Gordon William Arbuthnott
Eur J Neurosci. 2019 Jan 11. DOI: 10.1111/ejn.14344
Roger Marek, Li Xu, Robert K. P. Sullivan, Pankaj Sah
Nature Neuroscience (2018) 21(5):654-658. DOI: 10.1038
Miyamoto D, Hirai D, Fung CCA, Inutsuka A, Odagawa M, Suzuki T, Boehringer R, Adaikkan C, Matsubara C, Matsuki N, Fukai T, McHugh TJ, Yamanaka A, Murayama M
Science (2016) 352(6291): 1315-8. DOI: 10.1126
Isosaka T, Matsuo T, Yamaguchi T, Funabiki K, Nakanishi S, Kobayakawa R, Kobayakawa K
Cell (2015) 163(5):1153-64
Manita S, Suzuki T, Homma C, Matsumoto T, Odagawara M, Yamada K, Ota K, Matsubara C, Inutsuka A, Sato M, Ohkura M, Yamanaka A, Yanagawa Y, Nakai J, Hayashi Y, Larkum ME, Murayama M
Neuron (2015) 86:1304-16
Frequently Asked Questions
For which animals do you recommend the Teleopto wireless optical stimulation system?
The Teleopto receivers dimensions vary depending on the model you choose. However, with a weight of maximum 3.1g and a size of a penny, the receivers are suitable even for mice!
How can the trigger pulses be generated?
The remote controller accepts TTL trigger signals from a stimulator and sends the signals to the receiver. Synchronized light pulses are generated from the tip of the LED cannula. If you don’t own a stimulator, we can provide you with a pulse generator specifically developed for optogenetics.
Can I stimulate with more than 2 channels at the same time?
Yes, we offer a Teleopto wireless optical stimulation system to control up to 4 channels.
The receiver is capable of controlling 4 channel optogenetic stimulation independently, whereas the size and weight are comparable to the regular Teleopto receivers.
PC software defines stimulation parameters which are sent to the receiver via a transmitter box connected to the PC by USB. You can trigger the stimulation by software, 4 trigger buttons or 4 trigger in BNCs on the transmitter box.
The typical application of this device is for controlling bilateral two color stimulation in completely independent manner. However, we can provide any probe / cannula holding 4 LED sources on request.
What is the difference between the 2 colour LED cannula and the bilateral LED cannula? Can I stimulate with more than 2 channels at the same time?
Together with the 2-channel receiver, the 2 colour LED cannula allows stimulation by two different colours at the same position.
The bilateral LED cannula allows stimulation in 2 different locations. If coupled with a single channel receiver, it will stimulate both positions simultaneously. If coupled with a 2-channel receiver, it will stimulate both positions independently.
Please get in touch for more information!
Teleopto Wireless Optical Stimulation
The Teleopto wireless optical stimulation system is the ideal solution for activating opsins in freely behaving mouse or rat.