High-resolution deep tissue imaging without being highly invasive is difficult to achieve. Therefore, a new imaging method that allows non- or minimally-invasive image acquisition in vivo and in real time with sub-cellular spatial resolution, and at sub-second sampling rates is needed.
MCI now offers an exciting new imaging method that allows minimally- invasive image acquisition in vivo and in real time with sub-cellular spatial resolution – the MCI Pryer Endoscopic Objective (abbreviated as MCI Pryer). The MCI Pryer is a combined endoscopic objective and is compatible with any fluorescence microscopes, such as our CleverScope Motorised Microscope or current 2P (2 photon) microscopes from Olympus and Nikon. This state-of-the-art objective allows for deep tissue access and visualisation in anaesthetised animals, awake animals with head fixation and even chronic recordings with our custom-built adaptor. If you are involved in 2 photon microscopy and 2 photon imaging then this endoscopic objective may well be perfect for you.
Image quality: Both types of Pryer lens incorporate Plan Fluoro Achromatic NIR objective.
Compatibility: The Pryer lens is compatible with all upright fluorescence microscopes including bright field microscopes, confocal, 2P and super resolution microscopes.
Can be easily integrated into most (Olympus, Nikon, etc) current 2 photon microscopes, and giving excellent spatiotemporal resolution of <0.3μm
Minimally invasive recordings from deep tissues such as brain regions (e.g. hippocampus) using anesthetized animals or awake animals that are head-fixed
Provides access to any organ or tissue from the surface to deep structures
Potential for longitudinal studies
Record dynamic changes in vivo
Can be used to study drug uptake
Easily tailored to your application and adapt to your needs
Has the potential to be used in a broad-range of applications, for instance: oncology, molecular imaging, immunology, virology, botany, etc.
Frequently Asked Questions
How deep can the Pryer endoscopic objective image?
Can the Pryer endoscopic objective be used with a cannula?
Can you image neuronal activity in real time with the Pryer endoscopic objective?
Yes you can. The Pryer endoscopic objective is a great addition to your current imaging setup enabling you to image deep inside the brain and even enables you to observe activity in sub-cellular compartments, like dendrites, in real time.
Pryer Endoscopic Objective
The Pryer Endoscopic Objective is a powerful tool for deep tissue imaging at sub-micron resolution.