Mightex Polygon Systems

Mightex’s market-leading Polygon pattern illuminators provide precise spatiotemporal control of light with subcellular resolution
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Mightex’s market-leading Polygon pattern illuminators provide precise spatiotemporal control of light with subcellular resolution, making it the perfect illumination tool for life science research.

 

    • Any Shape, Size, Timing, and Colour
    • Simultaneous Multi-Region Illumination
    • Subcellular Resolution
    • Compatible with Any Microscope

The Polygon uses digital mirror device (DMD) technology to illuminate multiple regions simultaneously. A DMD is composed of hundreds of thousands of micro-mirrors that can be individually turned on to reflect light onto the sample. Thus, you can control each mirror to control the area(s) of illumination and create any number of different sized patterns. The Polygon DMD device can be mounted into the infinity-path of any microscope.

Polygon Digital Mirror Device

Mightex’s Digital Mirror Device (DMD) is inside their Polygon systems, which are compatible with every type of microscope. The DMD enables precise spatial illumination

 

New large DMD chip combined with interchangeable front tube optics enables larger field-of-view without comprising resolution and power.

Achieve better resolution with the Polygon 1000-G

The polygon 1000-G is a step up from the Polygon 400-G

 

Faster than Anything Else in the Market

Increased maximum frame rate means better temporal resolution for advanced physiologically-relevant experiments and virtually simultaneous 2-colour illumination of distinct ROIs.

Polygon 1000-G Frame rate

Polygon 1000-G offers 6,600 fps

Faster uploading time enables the Polygon1000 to perform real-time pattern illumination for closed-loop experiments (faster than HDMI refresh rates [60Hz/120Hz]).

Polygon 1000-G enables closed-loop experiments

Faster uploading time enables closed-loop control

 

More Power, Extra Flexibility

Large chip and improved optics enhances transmission efficiency enabling the Polygon1000 to achieve increased power density at the specimen level giving the researcher more room for intensity control.

Polygon 1000-G benefits from increased optical power density at the specimen level

Polygon 1000-G has 45% increased power density at specimen level compared to Polygon 400-G

Polygon1000-G

The Polygon1000-G is a flexible solution for patterned illumination, as this patterned illuminator can be used with any lightsource (350-700nm) that accepts a 3mm core lightguide. Thus, the Polygon1000-G provides future flexibility for different wavelengths and lightsources, depending on your application.

  • Wavelengths: 350-700nm
  • Lightsource: lightguide-coupled
  • Interchangeable front tubes available for fine resolution or large field-of-view

Key Applications: Neuroscience Optogenetics, Cell Biology Optogenetics, Photoactivation, Photoconversion

Mightex's Polygon 1000 1x Tube

Mightex’s Polygon 1000 1x Tube

 

Polygon1000-DL

The Polygon1000-DL is a flexible solution for large field of view or high-power patterned illumination applications, as this patterned illuminator can be used with any fiber-coupled lightsource (400-700nm), such as high-power lasers. This Polygon1000 model has been designed for high-power applications, such as in vivo optogenetics.

  • Wavelengths: 400-700nm
  • Lightsource: fiber-coupled
  • Interchangeable front tubes available for fine resolution or large field-of-view

Key Applications: In Vivo Optogenetics, Cortex-Wide Optogenetics, Photoactivation

Polygon1000 with 2x Front Tube

Polygon1000 with 2x Front Tube

Mightex’s PolyScan software platform is bundled with every Polygon to help you execute sophisticated patterned illumination experiments for your research. It provides:

  • Easy to use GUI to draw and define illumination patterns
  • Arrange sequences of illumination patterns
  • Define temporal illumination parameters for you experiments
  • Synchronize illumination patterns with other lab equipment

The Polygon enables scientists to precisely control where light will hit their sample. With subcellular resolution, the Polygon can illuminate any cellular feature in any shape or size. Our pattern editor allows the researcher to draw any pattern in true dimension or import an image of interest to project onto their sample, providing full spatial control of illumination.

Any shape illumination is possible

Polygon 1000-G can achieve any shape or pattern of illumination

 

Multi-Wavelength Illumination

The Polygon provides great flexibility when it comes down to wavelength selection. From UV to VIS/NIR range, the Polygon can project light of different colours suitable for your light-sensitive constructs.

Polygon 1000-G achieves multi-wavelength illumination

Illuminate different patterns with multiple wavelengths

 

Unlike a point scanner that sequentially illuminates a region-of-interest pixel by pixel, the Polygon projects all ON pixels simultaneously such that multiple regions-of-interest are illuminated at the exact same time.

 

Temporal & Intensity Control of Light Stimulation

Control the initiation, duration, and intensity of light stimulation patterns using the Polygon. Create different waveforms to control the light intensity and duration outputted from the Polygon.

Compatible with Any Microscope

The Polygon is designed to be coupled into the infinity space of any microscope model (Leica, Nikon, Olympus, Zeiss) with Mightex’s microscope-specific adapters. Please contact Mightex to discuss the different options available for coupling to a microscope.

Microscope polygon schematic to demonstrate compatibility

The Polygon is compatible with any microscope

 

Synchronize with Electrophysiology or Imaging Equipment

The Polygon comes with BNC connectorized TTL trigger input and output for easy synchronization with multiple lab equipment. Synchronize the Polygon with your motorized stage for field of view stitching, or synchronize your stimulation patterns with electrophysiology equipment.

 

Third-Party Camera Compatible

The Polygon works with any third-party camera. All the Polygon needs is access to the imaging acquisition window for calibration and pattern creation.

Third party camera compatible

Polygon 1000-G can be used with a third party camera

 

Microscope Adaptors

Mightex offers different microscope adapters for all inverted and upright microscopes. The microscope adapter is designed to couple the Polygon into the infinity-path of the microscope for patterned illumination capabilities.

Polygon Microscope Adaptor

Mightex offers different microscope adaptors for all inverted and upright microscopes.

Cannabinoid CB1 Receptors in the Amygdalar Cholecystokinin Glutamatergic Afferents to Nucleus Accumbens Modulate Depressive-Like Behavior – Nature Medicine (2019)

Shen C, Zheng D, Li K, Yang J, Pan H, Yu X, Fu J, Zhu Y, Sun Q, Tang M, Zhang Y, Sun P, Xie Y, Duan S, Hu H, & Li X

Application(s) : Neuroscience Optogenetics

 

Rostral and Caudal Ventral Tegmental Area GABAergic Inputs to Different Dorsal Raphe Neurons Participate in Opioid Dependence – Neuron (2019)

Li Y, Li C, Xi W, Jin S, Wu Z, Jiang P, Dong P, He X, Xu F, Duan S, Zhou Y, & Li X

Application(s) : Neuroscience Optogenetics

 

Bicistronic Construct for Optogenetic Prosthesis of Ganglion Cell Receptive Field of Degenerative Retina -Doklady Biochemistry and Biophysics (2019)

Petrovskaya LE, Roshchin MV, Smirnova GR, Kolotova DE, Balaban PM, Ostrovsky MA, & Malyshev AY

Application(s) : Neuroscience Optogenetics

 

Disparities in Short-Term Depression Among Prefrontal Cortex Synapses Sustain Persistent Activity in a Balanced Network – Cerebral Cortex (2019)

Yoon JY, Lee HR, Ho WK, & Lee SH

Application(s) : Neuroscience Optogenetics

 

Long-Lasting Response Changes in Deep Cerebellar Nuclei in vivo Correlate with Low-Frequency Oscillations – Frontiers in Cellular Neuroscience (2019)

Moscato L, Montagna I, De Propris L, Tritto S, Mapelli L, & D’Angelo E

Application(s) : Neuroscience Optogenetics

 

Signaling Dynamics Control Cell Fate in the Early Drosophila Embryo – Developmental Cell (2019)

Johnson HE & Toettcher JE

Application(s) : Cell Biology Optogenetics

 

Uneven Balance of Power Between Hypothalamic Peptidergic Neurons in the Control of Feeding – PNAS (2018)

Wei Q, Krolewski DM, Moore S, Kumar V, Li F, Martin B, Tomer R, Murphy GG, Deisseroth K, Watson Jr SJ, & Akil H

Application(s) : Neuroscience Optogenetics

 

Optogenetics Enables Real-Time Spatiotemporal Control Over Spiral Wave Dynamics in an Excitable Cardiac System – Elife (2018)

Majumder R, Feola I, Teplenin AS, de Vries AA, Panfilov AV, & Pijnappels D

Application(s) : Cardiac Optogenetics

 

Co-Transmission of Acetylcholine and GABA Regulates Hippocampal States – Nature Communications (2018)

Takacs VT, Cserep C, Schlingloff D, Posfai B, Szonyi A, Sos KE, Kornyei Z, Denes A, Gulyas AI, Freund TF, & Nyiri G

Application(s) : Neuroscience Optogenetics

 

VIP-Immunoreactive Interneurons Within Circuits of the Mouse Basolateral Amygdala – Journal of Neuroscience (2018)

Rhomberg T, Rovira-Esteban L, Vikor A, Paradiso E, Kremser C, Nagry-Pal P, Papp OI, Tasan R, Erdelyi F, Szabo G, Ferraguti F, & Hajos N

Application(s) : Neuroscience Optogenetics

 

Layer I Interneurons Sharpen Sensory Maps during Neonatal Development – Neuron (2018)

Che A, Babij R, Iannone AF, Fetcho RN, Ferrer M, Liston C, Fishell G, & De Marco Garcia NV

Application(s) : Neuroscience Optogenetics

Can I illuminate cells with multiple wavelengths?

Yes, you can. And you can illuminate multiple patterns with mutliple wavelengths.

How fast can I switch between illumination patterns?

The Polygon 1000 takes just 4 ms to switch between patterns. Meaning the user can perform closed-loop experiments if needed.

Is there a limit to the number of cells that can be illuminated?

Theoretically yes, but to achieve the limit you would need to optically stimulate the entire field of view. In practice, you can illuminate as many discreet cells of interest under your field of view as you need to.

Mightex Polygon Systems

Mightex’s market-leading Polygon pattern illuminators provide precise spatiotemporal control of light with subcellular resolution